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Human IgG1 Hinge Fragmentation as the Result of H(2)O(2)-mediated Radical Cleavage

Hinge cleavage of a recombinant human IgG1 antibody, generated during production in a Chinese hamster ovary cell culture, was observed in the purified material. The cleavage products could be reproduced by incubation of the antibody with H(2)O(2) and featured complementary ladders of the C- and N-te...

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Detalhes bibliográficos
Main Authors: Yan, Boxu, Yates, Zac, Balland, Alain, Kleemann, Gerd R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2790968/
https://ncbi.nlm.nih.gov/pubmed/19850927
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.064147
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