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Direct detection of DNA methylation during single-molecule, real-time sequencing
We describe the direct detection of DNA methylation, without bisulfite conversion, through single-molecule real-time (SMRT) sequencing. In SMRT sequencing, DNA polymerases catalyze the incorporation of fluorescently labeled nucleotides into complementary nucleic acid strands. The arrival times and d...
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| Hlavní autoři: | , , , , , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
2010
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2879396/ https://ncbi.nlm.nih.gov/pubmed/20453866 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1459 |
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