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Sensitive and specific single-molecule sequencing of 5-hydroxymethylcytosine
We describe strand-specific, base-resolution detection of 5-hydroxymethylcytosine (5-hmC) in genomic DNA with single-molecule sensitivity, combining a bioorthogonal, selective chemical labeling method of 5-hmC with single-molecule, real-time (SMRT) DNA sequencing. The chemical labeling not only allo...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3646335/ https://ncbi.nlm.nih.gov/pubmed/22101853 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1779 |
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