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Reduced seed region-based off-target activity with lentivirus-mediated RNAi

Along with silencing intended target genes, transfected siRNAs regulate numerous unintended transcripts through a mechanism in which the equivalent of a microRNA-like seed region in the siRNA recognizes complementary sequences in transcript 3′ UTRs. Amelioration of this off-target silencing would le...

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Bibliografski detalji
Glavni autori: Klinghoffer, Richard A., Magnus, Jill, Schelter, Janell, Mehaffey, Michele, Coleman, Casey, Cleary, Michele A.
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 2010
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2856882/
https://ncbi.nlm.nih.gov/pubmed/20348445
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1977810
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