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siRNA Specificity: RNAi Mechanisms and Strategies to Reduce Off-Target Effects

Short interfering RNAs (siRNAs) are processed from long double-stranded RNA (dsRNA), and a guide strand is selected and incorporated into the RNA-induced silencing complex (RISC). Within RISC, a member of the Argonaute protein family directly binds the guide strand and the siRNA guides RISC to fully...

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Bibliographic Details
Published in:Front Plant Sci
Main Authors: Neumeier, Julia, Meister, Gunter
Format: Artigo
Language:Inglês
Published: Frontiers Media S.A. 2021
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7876455/
https://ncbi.nlm.nih.gov/pubmed/33584737
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2020.526455
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