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siPools: highly complex but accurately defined siRNA pools eliminate off-target effects

Short interfering RNAs (siRNAs) are widely used as tool for gene inactivation in basic research and therapeutic applications. One of the major shortcomings of siRNA experiments are sequence-specific off-target effects. Such effects are largely unpredictable because siRNAs can affect partially comple...

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Detalhes bibliográficos
Main Authors: Hannus, Michael, Beitzinger, Michaela, Engelmann, Julia C., Weickert, Marie-Theresa, Spang, Rainer, Hannus, Stefan, Meister, Gunter
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2014
Assuntos:
RNA
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4081087/
https://ncbi.nlm.nih.gov/pubmed/24875475
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku480
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