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Efficient integration of transgenes into a defined locus in human embryonic stem cells
Random integration is one of the more straightforward methods to introduce a transgene into human embryonic stem (ES) cells. However, random integration may result in transgene silencing and altered cell phenotype due to insertional mutagenesis in undefined gene regions. Moreover, reliability of dat...
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| Main Authors: | , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2853137/ https://ncbi.nlm.nih.gov/pubmed/20071742 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp1234 |
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