Efficient integration of transgenes into a defined locus in human embryonic stem cells

Random integration is one of the more straightforward methods to introduce a transgene into human embryonic stem (ES) cells. However, random integration may result in transgene silencing and altered cell phenotype due to insertional mutagenesis in undefined gene regions. Moreover, reliability of dat...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Sakurai, Kenji, Shimoji, Miho, Tahimic, Candice G. T., Aiba, Kazuhiro, Kawase, Eihachiro, Hasegawa, Kouichi, Amagai, Yuji, Suemori, Hirofumi, Nakatsuji, Norio
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 2010
Pynciau:
Methods Online
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC2853137/
https://ncbi.nlm.nih.gov/pubmed/20071742
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp1234
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