Efficient and Accurate Homologous Recombination in hESCs and hiPSCs Using Helper-dependent Adenoviral Vectors

Low efficiencies of gene targeting via homologous recombination (HR) have limited basic research and applications using human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Here, we show highly and equally efficient gene knockout and knock-in at both transcriptionall...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Aizawa, Emi, Hirabayashi, Yuka, Iwanaga, Yuzuru, Suzuki, Keiichiro, Sakurai, Kenji, Shimoji, Miho, Aiba, Kazuhiro, Wada, Tamaki, Tooi, Norie, Kawase, Eihachiro, Suemori, Hirofumi, Nakatsuji, Norio, Mitani, Kohnosuke
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2012
Assuntos:
Original Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3277220/
https://ncbi.nlm.nih.gov/pubmed/22146343
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/mt.2011.266
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados