Efficient and Accurate Homologous Recombination in hESCs and hiPSCs Using Helper-dependent Adenoviral Vectors

Low efficiencies of gene targeting via homologous recombination (HR) have limited basic research and applications using human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Here, we show highly and equally efficient gene knockout and knock-in at both transcriptionall...

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Библиографические подробности
Главные авторы: Aizawa, Emi, Hirabayashi, Yuka, Iwanaga, Yuzuru, Suzuki, Keiichiro, Sakurai, Kenji, Shimoji, Miho, Aiba, Kazuhiro, Wada, Tamaki, Tooi, Norie, Kawase, Eihachiro, Suemori, Hirofumi, Nakatsuji, Norio, Mitani, Kohnosuke
Формат: Artigo
Язык:Inglês
Опубликовано: Nature Publishing Group 2012
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Original Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC3277220/
https://ncbi.nlm.nih.gov/pubmed/22146343
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/mt.2011.266
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