Efficient and Accurate Homologous Recombination in hESCs and hiPSCs Using Helper-dependent Adenoviral Vectors

Low efficiencies of gene targeting via homologous recombination (HR) have limited basic research and applications using human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Here, we show highly and equally efficient gene knockout and knock-in at both transcriptionall...

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Hlavní autoři: Aizawa, Emi, Hirabayashi, Yuka, Iwanaga, Yuzuru, Suzuki, Keiichiro, Sakurai, Kenji, Shimoji, Miho, Aiba, Kazuhiro, Wada, Tamaki, Tooi, Norie, Kawase, Eihachiro, Suemori, Hirofumi, Nakatsuji, Norio, Mitani, Kohnosuke
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group 2012
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Original Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3277220/
https://ncbi.nlm.nih.gov/pubmed/22146343
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/mt.2011.266
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