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Mutant APH(2″)-IIa Enzymes with Increased Activity against Amikacin and Isepamicin

Directed evolution by random PCR mutagenesis of the gene for the aminoglycoside 2″-IIa phosphotransferase generated R92H/D268N and N196D/D268N mutant enzymes, resulting in elevated levels of resistance to amikacin and isepamicin but not to other aminoglycoside antibiotics. Increases in the activitie...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Toth, Marta, Frase, Hilary, Chow, Joseph W., Smith, Clyde, Vakulenko, Sergei B.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Microbiology (ASM) 2010
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC2849353/
https://ncbi.nlm.nih.gov/pubmed/20145089
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.01444-09
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