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Tricistronic Overexpression of Cytochrome P450(cam), Putidaredoxin, and Putidaredoxin Reductase Provides a Useful Cell-based Catalytic System

The catalytic turnover of cytochrome P450(cam) from Pseudomonas putida requires two auxiliary reduction partners, putidaredoxin (Pd) and putidaredoxin reductase (PdR). We report the functional expression in Escherichia coli of tricistronic constructs consisting of P450(cam) encoded by the first cist...

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Bibliografiske detaljer
Main Authors: Kim, Donghak, Ortiz de Montellano, Paul R.
Format: Artigo
Sprog:Inglês
Udgivet: 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2844094/
https://ncbi.nlm.nih.gov/pubmed/19458919
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10529-009-0016-3
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