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Tricistronic Overexpression of Cytochrome P450(cam), Putidaredoxin, and Putidaredoxin Reductase Provides a Useful Cell-based Catalytic System
The catalytic turnover of cytochrome P450(cam) from Pseudomonas putida requires two auxiliary reduction partners, putidaredoxin (Pd) and putidaredoxin reductase (PdR). We report the functional expression in Escherichia coli of tricistronic constructs consisting of P450(cam) encoded by the first cist...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2009
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2844094/ https://ncbi.nlm.nih.gov/pubmed/19458919 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10529-009-0016-3 |
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