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Tricistronic Overexpression of Cytochrome P450(cam), Putidaredoxin, and Putidaredoxin Reductase Provides a Useful Cell-based Catalytic System

The catalytic turnover of cytochrome P450(cam) from Pseudomonas putida requires two auxiliary reduction partners, putidaredoxin (Pd) and putidaredoxin reductase (PdR). We report the functional expression in Escherichia coli of tricistronic constructs consisting of P450(cam) encoded by the first cist...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Kim, Donghak, Ortiz de Montellano, Paul R.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2009
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2844094/
https://ncbi.nlm.nih.gov/pubmed/19458919
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10529-009-0016-3
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