Stable genetic transformation of intact Nicotiana cells by the particle bombardment process

We show that the genetic transformation of Nicotiana tabacum can be achieved by bombarding intact cells and tissues with DNA-coated particles. Leaves or suspension culture cells were treated with tungsten microprojectiles carrying plasmid DNA containing a neomycin phosphotransferase gene. Callus har...

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Argitaratua izan da:	Proc Natl Acad Sci U S A
Egile Nagusiak:	Klein, Theodore M., Harper, Elisabeth C., Svab, Zora, Sanford, John C., Fromm, Michael E., Maliga, Pal
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	National Academy of Sciences 1988
Gaiak:	Biological Sciences: Botany
Sarrera elektronikoa:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282486/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16593993/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.22.8502
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