Stable genetic transformation of intact Nicotiana cells by the particle bombardment process

We show that the genetic transformation of Nicotiana tabacum can be achieved by bombarding intact cells and tissues with DNA-coated particles. Leaves or suspension culture cells were treated with tungsten microprojectiles carrying plasmid DNA containing a neomycin phosphotransferase gene. Callus har...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Klein, Theodore M., Harper, Elisabeth C., Svab, Zora, Sanford, John C., Fromm, Michael E., Maliga, Pal
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1988
Soggetti:	Biological Sciences: Botany
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282486/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16593993/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.22.8502
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