Stable genetic transformation of intact Nicotiana cells by the particle bombardment process

We show that the genetic transformation of Nicotiana tabacum can be achieved by bombarding intact cells and tissues with DNA-coated particles. Leaves or suspension culture cells were treated with tungsten microprojectiles carrying plasmid DNA containing a neomycin phosphotransferase gene. Callus har...

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Dades bibliogràfiques

Publicat a:	Proc Natl Acad Sci U S A
Autors principals:	Klein, Theodore M., Harper, Elisabeth C., Svab, Zora, Sanford, John C., Fromm, Michael E., Maliga, Pal
Format:	Artigo
Idioma:	Inglês
Publicat:	National Academy of Sciences 1988
Matèries:	Biological Sciences: Botany
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282486/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16593993/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.22.8502
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