Stable genetic transformation of intact Nicotiana cells by the particle bombardment process

We show that the genetic transformation of Nicotiana tabacum can be achieved by bombarding intact cells and tissues with DNA-coated particles. Leaves or suspension culture cells were treated with tungsten microprojectiles carrying plasmid DNA containing a neomycin phosphotransferase gene. Callus har...

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Detalles Bibliográficos

Publicado en:	Proc Natl Acad Sci U S A
Principais autores:	Klein, Theodore M., Harper, Elisabeth C., Svab, Zora, Sanford, John C., Fromm, Michael E., Maliga, Pal
Formato:	Artigo
Idioma:	Inglês
Publicado:	National Academy of Sciences 1988
Assuntos:	Biological Sciences: Botany
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282486/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16593993/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.22.8502
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