Stable genetic transformation of intact Nicotiana cells by the particle bombardment process

We show that the genetic transformation of Nicotiana tabacum can be achieved by bombarding intact cells and tissues with DNA-coated particles. Leaves or suspension culture cells were treated with tungsten microprojectiles carrying plasmid DNA containing a neomycin phosphotransferase gene. Callus har...

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Bibliografiske detaljer
Main Authors: Klein, Theodore M., Harper, Elisabeth C., Svab, Zora, Sanford, John C., Fromm, Michael E., Maliga, Pal
Format: Artigo
Sprog:Inglês
Udgivet: 1988
Fag:
Biological Sciences: Botany
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC282486/
https://ncbi.nlm.nih.gov/pubmed/16593993
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