Stable genetic transformation of intact Nicotiana cells by the particle bombardment process

We show that the genetic transformation of Nicotiana tabacum can be achieved by bombarding intact cells and tissues with DNA-coated particles. Leaves or suspension culture cells were treated with tungsten microprojectiles carrying plasmid DNA containing a neomycin phosphotransferase gene. Callus har...

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Λεπτομέρειες βιβλιογραφικής εγγραφής

Εκδόθηκε σε:	Proc Natl Acad Sci U S A
Κύριοι συγγραφείς:	Klein, Theodore M., Harper, Elisabeth C., Svab, Zora, Sanford, John C., Fromm, Michael E., Maliga, Pal
Μορφή:	Artigo
Γλώσσα:	Inglês
Έκδοση:	National Academy of Sciences 1988
Θέματα:	Biological Sciences: Botany
Διαθέσιμο Online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282486/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16593993/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.22.8502
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