Activation of R235A Mutant Orotidine 5'-Monophosphate Decarboxylase by the Guanidinium Cation: Effective Molarity of the Cationic Side Chain of Arg-235

The R235A mutation at yeast orotidine 5'-monophosphate decarboxylase (OMPDC) results in a 1300-fold increase in K(m) and a 14-fold decrease in k(cat) for decarboxylation of orotidine 5'-monophosphate, corresponding to a 5.8 kcal/mol destabilization of the transition state. There is strong...

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Main Authors: Barnett, Shonoi A., Amyes, Tina L., Wood, B. McKay, Gerlt, John A., Richard, John P.
Formato: Artigo
Idioma:Inglês
Publicado: 2010
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2819103/
https://ncbi.nlm.nih.gov/pubmed/20050635
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi902174q
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