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Binding and Cleavage Specificities of Human Argonaute2

The endonuclease Argonaute2 (Ago2) mediates the degradation of the target mRNA within the RNA-induced silencing complex. We determined the binding and cleavage properties of recombinant human Ago2. Human Ago2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA d...

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Bibliografische gegevens
Hoofdauteurs: Lima, Walt F., Wu, Hongjiang, Nichols, Josh G., Sun, Hong, Murray, Heather M., Crooke, Stanley T.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Biochemistry and Molecular Biology 2009
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2758002/
https://ncbi.nlm.nih.gov/pubmed/19625255
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.010835
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