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Targeted Manipulation of Mammalian Genomes using Designed Zinc Finger Nucleases
Targeted introduction of a double-stranded break (DSB) using designer zinc finger nucleases (ZFNs) in mammalian cells greatly enhances gene targeting - homologous recombination (HR) at a chosen endogenous target gene, which otherwise is limited by low spontaneous rate of HR. Here, we report that eff...
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| Main Authors: | , , , , , , , |
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| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
2009
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2744961/ https://ncbi.nlm.nih.gov/pubmed/19635463 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2009.07.112 |
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