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Binding of two zinc finger nuclease monomers to two specific sites is required for effective double-strand DNA cleavage

Custom-designed zinc finger nucleases (ZFNs) are becoming powerful tools in gene targeting—the process of replacing a gene within a genome by homologous recombination. Here, we have studied the DNA cleavage by one such ZFN, ΔQNK-FN, in order to gain insight into how ZFNs cleave DNA and how two inver...

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Detalhes bibliográficos
Main Authors: Mani, Mala, Smith, Jeff, Kandavelou, Karthikeyan, Berg, Jeremy M., Chandrasegaran, Srinivasan
Formato: Artigo
Idioma:Inglês
Publicado em: 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4170802/
https://ncbi.nlm.nih.gov/pubmed/16043120
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2005.07.021
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