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Substrate-Triggered Formation and Remarkable Stability of the C-H-Cleaving Chloroferryl Intermediate in the Aliphatic Halogenase, SyrB2

Aliphatic halogenases activate O(2), cleave α-ketoglutarate (αKG) to CO(2) and succinate, and form haloferryl [X-Fe(IV)=O; X = Cl, Br] complexes that cleave aliphatic C-H bonds to install halogens during the biosynthesis of natural products by non-ribosomal peptide synthetases (NRPSs). For the relat...

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Bibliografiske detaljer
Main Authors: Matthews, Megan L., Krest, Courtney M., Barr, Eric W., Vaillancourt, Frédéric H., Walsh, Christopher T., Green, Michael T., Krebs, Carsten, Bollinger, J. Martin
Format: Artigo
Sprog:Inglês
Udgivet: 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2684568/
https://ncbi.nlm.nih.gov/pubmed/19245217
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi900109z
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