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Surveying polypeptide and protein domain conformation and association with FlAsH and ReAsH

Recombinant polypeptides and protein domains containing two cysteine pairs located distal in primary sequence but proximal in the native folded or assembled state are labeled selectively in vitro and in mammalian cells using the profluorescent biarsenical reagents FlAsH-EDT(2) and ReAsH-EDT(2). This...

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Détails bibliographiques
Auteurs principaux: Luedtke, Nathan W, Dexter, Rachel J, Fried, Daniel B, Schepartz, Alanna
Format: Artigo
Langue:Inglês
Publié: 2007
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2679367/
https://ncbi.nlm.nih.gov/pubmed/17982447
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.2007.49
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