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Surveying polypeptide and protein domain conformation and association with FlAsH and ReAsH

Recombinant polypeptides and protein domains containing two cysteine pairs located distal in primary sequence but proximal in the native folded or assembled state are labeled selectively in vitro and in mammalian cells using the profluorescent biarsenical reagents FlAsH-EDT(2) and ReAsH-EDT(2). This...

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Main Authors: Luedtke, Nathan W, Dexter, Rachel J, Fried, Daniel B, Schepartz, Alanna
Formato: Artigo
Idioma:Inglês
Publicado: 2007
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2679367/
https://ncbi.nlm.nih.gov/pubmed/17982447
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.2007.49
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