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Surveying polypeptide and protein domain conformation and association with FlAsH and ReAsH

Recombinant polypeptides and protein domains containing two cysteine pairs located distal in primary sequence but proximal in the native folded or assembled state are labeled selectively in vitro and in mammalian cells using the profluorescent biarsenical reagents FlAsH-EDT(2) and ReAsH-EDT(2). This...

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Hlavní autoři: Luedtke, Nathan W, Dexter, Rachel J, Fried, Daniel B, Schepartz, Alanna
Médium: Artigo
Jazyk:Inglês
Vydáno: 2007
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2679367/
https://ncbi.nlm.nih.gov/pubmed/17982447
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.2007.49
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