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Preparation of the membrane-permeant biarsenicals, FlAsH-EDT(2) and ReAsH-EDT(2) for fluorescent labeling of tetracysteine-tagged proteins

The membrane-permeant, fluorogenic biarsenicals, FlAsH-EDT(2) and ReAsH-EDT(2) can be prepared in good yields by a straightforward two-step procedure from the inexpensive precursor dyes, fluorescein and resorufin respectively. Handling of toxic reagents such as arsenic trichloride is minimized so th...

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Detalles Bibliográficos
Main Authors: Adams, Stephen R., Tsien, Roger Y.
Formato: Artigo
Idioma:Inglês
Publicado: 2008
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2843588/
https://ncbi.nlm.nih.gov/pubmed/18772880
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2008.144
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