Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases

We report very high gene targeting frequencies in Drosophila by direct embryo injection of mRNAs encoding specific zinc-finger nucleases (ZFNs). Both local mutagenesis via nonhomologous end joining (NHEJ) and targeted gene replacement via homologous recombination (HR) have been achieved in up to 10%...

Descripció completa

Guardat en:
Dades bibliogràfiques
Autors principals: Beumer, Kelly J., Trautman, Jonathan K., Bozas, Ana, Liu, Ji-Long, Rutter, Jared, Gall, Joseph G., Carroll, Dana
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2008
Matèries:
Biological Sciences
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2604940/
https://ncbi.nlm.nih.gov/pubmed/19064913
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0810475105
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!

Ítems similars