Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases

We report very high gene targeting frequencies in Drosophila by direct embryo injection of mRNAs encoding specific zinc-finger nucleases (ZFNs). Both local mutagenesis via nonhomologous end joining (NHEJ) and targeted gene replacement via homologous recombination (HR) have been achieved in up to 10%...

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Bibliografiske detaljer
Main Authors: Beumer, Kelly J., Trautman, Jonathan K., Bozas, Ana, Liu, Ji-Long, Rutter, Jared, Gall, Joseph G., Carroll, Dana
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2008
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2604940/
https://ncbi.nlm.nih.gov/pubmed/19064913
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0810475105
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