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Genetic Analysis of Zinc-Finger Nuclease-Induced Gene Targeting in Drosophila
Using zinc-finger nucleases (ZFNs) to cleave the chromosomal target, we have achieved high frequencies of gene targeting in the Drosophila germline. Both local mutagenesis through nonhomologous end joining (NHEJ) and gene replacement via homologous recombination (HR) are stimulated by target cleavag...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Genetics Society of America
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2710147/ https://ncbi.nlm.nih.gov/pubmed/19380480 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.109.101329 |
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