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Genetic Analysis of Zinc-Finger Nuclease-Induced Gene Targeting in Drosophila

Using zinc-finger nucleases (ZFNs) to cleave the chromosomal target, we have achieved high frequencies of gene targeting in the Drosophila germline. Both local mutagenesis through nonhomologous end joining (NHEJ) and gene replacement via homologous recombination (HR) are stimulated by target cleavag...

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Autors principals: Bozas, Ana, Beumer, Kelly J., Trautman, Jonathan K., Carroll, Dana
Format: Artigo
Idioma:Inglês
Publicat: Genetics Society of America 2009
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2710147/
https://ncbi.nlm.nih.gov/pubmed/19380480
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.109.101329
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