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Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases
We report very high gene targeting frequencies in Drosophila by direct embryo injection of mRNAs encoding specific zinc-finger nucleases (ZFNs). Both local mutagenesis via nonhomologous end joining (NHEJ) and targeted gene replacement via homologous recombination (HR) have been achieved in up to 10%...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2604940/ https://ncbi.nlm.nih.gov/pubmed/19064913 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0810475105 |
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