Targeted Mutagenesis of Burkholderia thailandensis and Burkholderia pseudomallei through Natural Transformation of PCR Fragments

Burkholderia pseudomallei is the causative agent of melioidosis, an overwhelming, rapidly fatal septic infection, and B. thailandensis is a closely related, less virulent species. Both organisms are naturally competent for DNA transformation, and this report describes a procedure exploiting this pro...

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Detalles Bibliográficos
Autores principales: Thongdee, Metawee, Gallagher, Larry A., Schell, Mark, Dharakul, Tararaj, Songsivilai, Sirirurg, Manoil, Colin
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology (ASM) 2008
Materias:
Methods
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2394929/
https://ncbi.nlm.nih.gov/pubmed/18310423
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00030-08
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