Targeted Mutagenesis of Burkholderia thailandensis and Burkholderia pseudomallei through Natural Transformation of PCR Fragments

Burkholderia pseudomallei is the causative agent of melioidosis, an overwhelming, rapidly fatal septic infection, and B. thailandensis is a closely related, less virulent species. Both organisms are naturally competent for DNA transformation, and this report describes a procedure exploiting this pro...

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Main Authors: Thongdee, Metawee, Gallagher, Larry A., Schell, Mark, Dharakul, Tararaj, Songsivilai, Sirirurg, Manoil, Colin
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 2008
Teme:
Methods
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2394929/
https://ncbi.nlm.nih.gov/pubmed/18310423
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00030-08
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