Knock-out and pull-out recombineering protocols for naturally transformable Burkholderia thailandensis and Burkholderia pseudomallei

Phage λ Red proteins are powerful tools for pulling- and knocking-out chromosomal fragments but have been limited to the γ-proteobacteria. Procedures are described here to easily knock-out (KO) and pull-out (PO) chromosomal DNA fragments from naturally transformable Burkholderia thailandensis and Bu...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Päätekijät: Kang, Yun, Norris, Michael H., Wilcox, Bruce A., Tuanyok, Apichai, Keim, Paul S., Hoang, Tung T.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2011
Aiheet:
Article
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC3564556/
https://ncbi.nlm.nih.gov/pubmed/21738123
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.346
Tagit: Lisää tagi
Ei tageja, Lisää ensimmäinen tagi!

Samankaltaisia teoksia