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Knock-out and pull-out recombineering protocols for naturally transformable Burkholderia thailandensis and Burkholderia pseudomallei
Phage λ Red proteins are powerful tools for pulling- and knocking-out chromosomal fragments but have been limited to the γ-proteobacteria. Procedures are described here to easily knock-out (KO) and pull-out (PO) chromosomal DNA fragments from naturally transformable Burkholderia thailandensis and Bu...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3564556/ https://ncbi.nlm.nih.gov/pubmed/21738123 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.346 |
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