Targeted Mutagenesis of Burkholderia thailandensis and Burkholderia pseudomallei through Natural Transformation of PCR Fragments

Burkholderia pseudomallei is the causative agent of melioidosis, an overwhelming, rapidly fatal septic infection, and B. thailandensis is a closely related, less virulent species. Both organisms are naturally competent for DNA transformation, and this report describes a procedure exploiting this pro...

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Detalhes bibliográficos
Main Authors: Thongdee, Metawee, Gallagher, Larry A., Schell, Mark, Dharakul, Tararaj, Songsivilai, Sirirurg, Manoil, Colin
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2008
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2394929/
https://ncbi.nlm.nih.gov/pubmed/18310423
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00030-08
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