Targeted Mutagenesis of Burkholderia thailandensis and Burkholderia pseudomallei through Natural Transformation of PCR Fragments

Burkholderia pseudomallei is the causative agent of melioidosis, an overwhelming, rapidly fatal septic infection, and B. thailandensis is a closely related, less virulent species. Both organisms are naturally competent for DNA transformation, and this report describes a procedure exploiting this pro...

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Détails bibliographiques
Auteurs principaux: Thongdee, Metawee, Gallagher, Larry A., Schell, Mark, Dharakul, Tararaj, Songsivilai, Sirirurg, Manoil, Colin
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 2008
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Methods
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2394929/
https://ncbi.nlm.nih.gov/pubmed/18310423
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00030-08
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