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Sequence-Defined Transposon Mutant Library of Burkholderia thailandensis

We constructed a near-saturation transposon mutant library for Burkholderia thailandensis, a low-virulence surrogate for the causative agent of melioidosis (Burkholderia pseudomallei). A primary set of nearly 42,000 unique mutants (~7.5 mutants/gene) was generated using transposon Tn5 derivatives. T...

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Autores principales: Gallagher, Larry A., Ramage, Elizabeth, Patrapuvich, Rapatbhorn, Weiss, Eli, Brittnacher, Mitch, Manoil, Colin
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society of Microbiology 2013
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3870259/
https://ncbi.nlm.nih.gov/pubmed/24194535
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mBio.00604-13
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