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Mutational analysis reveals E. coli oriC interacts with both DnaA-ATP and DnaA-ADP during pre-RC assembly

Prior to initiating DNA synthesis, E. coli oriC switches from ORC, comprising initiator DnaA bound at three high affinity sites, to pre-RC, when additional DnaA molecules interact with low affinity sites. Two types of low affinity sites exist; R boxes that bind DnaA-ATP and DnaA-ADP with equal affin...

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Autori principali: Grimwade, Julia E., Torgue, Julien J-C., McGarry, Kevin C., Rozgaja, Tanya, Enloe, Sareena T., Leonard, Alan C.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2007
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2391298/
https://ncbi.nlm.nih.gov/pubmed/17850252
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1365-2958.2007.05930.x
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