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An efficient in vitro translation system from mammalian cells lacking the translational inhibition caused by eIF2 phosphorylation

In vitro translation systems are used to investigate translational mechanisms and to synthesize proteins for characterization. Most available mammalian cell-free systems have reduced efficiency due to decreased translation initiation caused by phosphorylation of the initiation factor eIF2α on Ser51....

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Bibliographische Detailangaben
Hauptverfasser: Zeenko, Vladimir V., Wang, Chuanping, Majumder, Mithu, Komar, Anton A., Snider, Martin D., Merrick, William C., Kaufman, Randal J., Hatzoglou, Maria
Format: Artigo
Sprache:Inglês
Veröffentlicht: Cold Spring Harbor Laboratory Press 2008
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2248251/
https://ncbi.nlm.nih.gov/pubmed/18230759
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.825008
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