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An efficient in vitro translation system from mammalian cells lacking the translational inhibition caused by eIF2 phosphorylation
In vitro translation systems are used to investigate translational mechanisms and to synthesize proteins for characterization. Most available mammalian cell-free systems have reduced efficiency due to decreased translation initiation caused by phosphorylation of the initiation factor eIF2α on Ser51....
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| 主要な著者: | , , , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Cold Spring Harbor Laboratory Press
2008
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2248251/ https://ncbi.nlm.nih.gov/pubmed/18230759 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.825008 |
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