An efficient in vitro translation system from mammalian cells lacking the translational inhibition caused by eIF2 phosphorylation

In vitro translation systems are used to investigate translational mechanisms and to synthesize proteins for characterization. Most available mammalian cell-free systems have reduced efficiency due to decreased translation initiation caused by phosphorylation of the initiation factor eIF2α on Ser51....

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Detalhes bibliográficos
Main Authors: Zeenko, Vladimir V., Wang, Chuanping, Majumder, Mithu, Komar, Anton A., Snider, Martin D., Merrick, William C., Kaufman, Randal J., Hatzoglou, Maria
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2008
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Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2248251/
https://ncbi.nlm.nih.gov/pubmed/18230759
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.825008
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