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Promoting motions in enzyme catalysis probed by pressure studies of kinetic isotope effects
Use of the pressure dependence of kinetic isotope effects, coupled with a study of their temperature dependence, as a probe for promoting motions in enzymatic hydrogen-tunneling reactions is reported. Employing morphinone reductase as our model system and by using stopped-flow methods, we measured t...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
2007
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1766415/ https://ncbi.nlm.nih.gov/pubmed/17202258 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0608408104 |
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