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Probing active site geometry using high pressure and secondary isotope effects in an enzyme-catalysed ‘deep’ H-tunnelling reaction

We report the first study of the effects of hydrostatic pressure on α-2° KIEs for an enzyme-catalysed H-transfer reaction that occurs by ‘deep’ tunnelling. High pressure causes a significant decrease in the observed α-2° KIE on the pre-steady-state hydride transfer from NADH to FMN in the flavoprote...

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Bibliografiske detaljer
Main Authors: Hay, Sam, Pudney, Christopher R., Sutcliffe, Michael J., Scrutton, Nigel S.
Format: Artigo
Sprog:Inglês
Udgivet: 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2948560/
https://ncbi.nlm.nih.gov/pubmed/20890464
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/poc.1653
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