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Discrimination of extended-spectrum beta-lactamases by a novel nitrocefin competition assay.

We describe a nitrocefin competition assay for determining inhibition profiles as a useful adjunct to existing biochemical methods for the discrimination of beta-lactamases. The hydrolysis rate of nitrocefin was measured with a plate photometer as the change in A480 over 45 min in the presence of 17...

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Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Antimicrob Agents Chemother
Egile Nagusiak: Papanicolaou, G A, Medeiros, A A
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: American Society for Microbiology (ASM) 1990
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC172021/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2073109/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.34.11.2184
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