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Discrimination of extended-spectrum beta-lactamases by a novel nitrocefin competition assay.

We describe a nitrocefin competition assay for determining inhibition profiles as a useful adjunct to existing biochemical methods for the discrimination of beta-lactamases. The hydrolysis rate of nitrocefin was measured with a plate photometer as the change in A480 over 45 min in the presence of 17...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Papanicolaou, G A, Medeiros, A A
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1990
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC172021/
https://ncbi.nlm.nih.gov/pubmed/2073109
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