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Discrimination of extended-spectrum beta-lactamases by a novel nitrocefin competition assay.

We describe a nitrocefin competition assay for determining inhibition profiles as a useful adjunct to existing biochemical methods for the discrimination of beta-lactamases. The hydrolysis rate of nitrocefin was measured with a plate photometer as the change in A480 over 45 min in the presence of 17...

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Hlavní autoři: Papanicolaou, G A, Medeiros, A A
Médium: Artigo
Jazyk:Inglês
Vydáno: 1990
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC172021/
https://ncbi.nlm.nih.gov/pubmed/2073109
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