Discrimination of extended-spectrum beta-lactamases by a novel nitrocefin competition assay.
We describe a nitrocefin competition assay for determining inhibition profiles as a useful adjunct to existing biochemical methods for the discrimination of beta-lactamases. The hydrolysis rate of nitrocefin was measured with a plate photometer as the change in A480 over 45 min in the presence of 17...
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| Udgivet i: | Antimicrob Agents Chemother |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1990
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC172021/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2073109/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.34.11.2184 |
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