Discrimination of extended-spectrum beta-lactamases by a novel nitrocefin competition assay.
We describe a nitrocefin competition assay for determining inhibition profiles as a useful adjunct to existing biochemical methods for the discrimination of beta-lactamases. The hydrolysis rate of nitrocefin was measured with a plate photometer as the change in A480 over 45 min in the presence of 17...
Guardat en:
| Publicat a: | Antimicrob Agents Chemother |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1990
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC172021/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2073109/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.34.11.2184 |
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