Discrimination of extended-spectrum beta-lactamases by a novel nitrocefin competition assay.

We describe a nitrocefin competition assay for determining inhibition profiles as a useful adjunct to existing biochemical methods for the discrimination of beta-lactamases. The hydrolysis rate of nitrocefin was measured with a plate photometer as the change in A480 over 45 min in the presence of 17...

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Veröffentlicht in:	Antimicrob Agents Chemother
Hauptverfasser:	Papanicolaou, G A, Medeiros, A A
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1990
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC172021/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2073109/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.34.11.2184
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